DHX58 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Rat liver Verified Samples in IHC:Human liver cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human DHX58 |
| Abbre | DHX58 |
| Synonyms | D11LGP2;D11lgp2e;DEXH (Asp Glu X His) box polypeptide 58;DEXH box polypeptide 58;DHX 58;DHX58;DHX58;LGP 2;LGP2;Ortholog of mouse D11lgp2;Probable ATP dependent helicase LGP2;Probable ATP dependent RNA helicase DHX58;Probable ATP-dependent helicase LGP2;Probable ATP-dependent RNA helicase DHX58;Protein D11Lgp2 homolog;RIG-I-like receptor LGP2;RLR;RNA helicase LGP2抗体 |
| Swissprot | |
| Calculated MW | 77 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. |
| Concentration | 1.02 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Immunology |
| Conjugation | Unconjugated |
| Background | Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate. |
Western blot analysis of Rat liver tissue lysate using DHX58 Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using DHX58 Polyclonal Antibody at dilution of 1:60(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
