Enhanced Cell Counting Kit 8 (WST-8/CCK8)
中文名称:增强型细胞活力检测试剂盒(CCK-8)
Price:
- 应用: 细胞活力/细胞增殖/细胞毒性
检测原理 | WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐),在电子载体存在的情况下,WST-8被线粒体内的脱氢酶还原生成水溶性的橙黄色formazan,生成的formazan的量与活细胞数量成正比,通过检测在450 nm处的吸光度,可以间接计算活细胞的量。 |
检测方法 | 比色法 |
样本类型 | 细胞 |
检测仪器 | 酶标仪 |
保存温度 | 该产品可在 2~8°C或-20°C避光条件下保存24个月。 |
有效期 | 24个月 |
运输条件 | 冰袋 |
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E-CK-A211 | Annexin V-FITC/PI Apoptosis Kit | 200Assays , 100Assays , 50Assays , 20Assays |
E-CK-A301 | Mitochondrial Membrane Potential Assay Kit (with JC-1) | 100Assays , 50Assays , 20Assays |
E-CK-A320 | One-step TUNEL In Situ Apoptosis Kit (Green, FITC) | 100Assays , 50Assays , 20Assays |
E-CK-A341 | MTT Cell Proliferation and Cytotoxicity Assay Kit | 1000Assays , 500Assays |
Q1:流式抗体实验结果的数据不满意/ Annexin V数据不会分析,能帮我分析下吗?
对于使用我司产品的客户,我们提供免费数据分析服务。您可以添加我们流式技术支持的企业微信,并将FCS格式原始数据、细胞样本(具体什么样本、如何处理)、实验分组(空白管、单染管、全染管、每个样本染色了哪些抗体)、流式操作步骤(细胞悬液体积、细胞个数,抗体用量、染色时间和温度)等信息发送我们技术支持同事。
Q2:含有抗荧光淬灭的封闭剂(含DAPI)可以用来封片吗?
可以用来封片,如果用的话就不用我们试剂盒里面自带的DAPI染料,但是由于DAPI孵育后没有清洗,所以会造成较高的背景。
Q3:细菌样本能否用Tunel、Annexin V检测?
老师您好,我们的验证都是在哺乳动物细胞上进行的,细菌的细胞膜结构和哺乳动物细胞膜结构存在差异,这些产品不一定能够适用,建议老师查询文献或者进行预实验来确认。