ENO1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Hela,A431,HepG2,Mouse brain,Mouse liver,Mouse pancreas,Human fetal brain Verified Samples in IHC:Mouse brain,Human thyroid cancer,Human kidney |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human ENO1 |
| Abbre | ENO1 |
| Synonyms | 2 phospho D glycerate hydro lyase;2-phospho-D-glycerate hydro-lyase;Alpha enolase;Alpha enolase like 1;Alpha-enolase;C myc promoter binding protein;C-myc promoter-binding protein;EC 4.2.1.11;eno1;ENO1L1;ENOA;Enolase 1 (alpha);Enolase 1 (alpha) like 1;Enolase 1;Enolase alpha;MBP 1;MBP-1;MBP1;MBPB1;MPB 1;MPB-1;MPB1;MYC promoter binding protein 1;NNE;Non neural enolase;Non-neural enolase;Phosphopyruvate hydratase;Plasminogen binding protein;Plasminogen-binding protein;PPH;Tau crystallin |
| Swissprot | |
| Calculated MW | 47kDa |
| Cellular Localization | Nucleus and Cytoplasm. Cell membrane. Cytoplasm>myofibril>sarcomere>M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line. |
| Concentration | 0.6 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signal Transduction |
| Conjugation | Unconjugated |
| Background | This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. |
Western Blot analysis of Hela, A431 and hepG2 cell, Mouse brain and liver tissue, Mouse pancreas and Human fetal brain tissue using ENO1 Polyclonal Antibody at dilution of 1:700
Immunohistochemistry of paraffin-embedded Mouse brain using ENO1 Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ENO1 Polyclonal Antibody at dilution of 1:60
Immunohistochemistry of paraffin-embedded Human Kidney using ENO1 Polyclonal Antibody at dilution of 1:60(Elabscience Product Detected by Lifespan).
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
