HLA-DRB1 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Human spleen,A375 Verified Samples in IHC:Human tonsil,Human cervical cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:300 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human HLA-DRB1 |
| Abbre | HLA-DRB1 |
| Synonyms | MHC Class II HLA-DR Beta 1 Chain;Human Leucocyte Antigen DRB1;MHC Class II Antigen DRB1*10;MHC Class II Antigen DRB1*11;MHC Class II Antigen DRB1*12;MHC Class II Antigen DRB1*13;MHC Class II Antigen DRB1*14;MHC Class II Antigen DRB1*15;MHC Class II Antigen DRB1*16;MHC Class II Antigen DRB1*1;MHC Class II Antigen DRB1*3;MHC Class II Antigen DRB1*4;MHC Class II Antigen DRB1*7;MHC Class II Antigen DRB1*8;MHC Class II Antigen DRB1*9;Lymphocyte Antigen DRB1c抗体 |
| Swissprot | |
| Calculated MW | 30 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Lysosome, Membrane, MHC II |
| Concentration | 1.14 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Immunology |
| Conjugation | Unconjugated |
| Background | HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. |
Western blot analysis of Human spleen tissue and A375 cell lysates using HLA-DRB1 Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human tonsil tissue using HLA-DRB1 Polyclonal Antibody at dilution of 1:65(×200)
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using HLA-DRB1 Polyclonal Antibody at dilution of 1:65(×200)
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