LFNG Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse; Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:K562,HT29 Verified Samples in IHC:Human tonsil,Human liver cancer |
| Dilution | WB 1:500-1:2000;IHC 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human LFNG |
| Abbre | LFNG |
| Synonyms | 3-N-acetylglucosaminyltransferase lunatic fringe;Beta-1;Beta-13-N-acetylglucosaminyltransferase lunatic fringe;lfng;LFNG;O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase;SCDO3抗体 |
| Swissprot | |
| Calculated MW | 42 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Golgi apparatus membrane. |
| Concentration | 1.08 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Developmental Biology; Neuroscience; Stem Cells |
| Conjugation | Unconjugated |
| Background | This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. |
Western blot analysis of K562 and HT-29 cell lysates using LFNG Polyclonal Antibody at dilution of 1:900
Immunohistochemistry of paraffin-embedded Human tonsil tissue using LFNG Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using LFNG Polyclonal Antibody at dilution of 1:60(×200)
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| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
