TPPP Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Human brain Verified Samples in IHC:Human liver cancer,Human thyroid cancer |
| Dilution | WB 1:500-1:2000;IHC 1:25-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Full length fusion protein |
| Abbre | TPPP |
| Synonyms | 25 kDa brain specific protein ;25 kDa brain-specific protein;Brain specific protein p25 alpha;Glycogen synthase kinase 3 (GSK3) inhibitor p24;OTTHUMP00000161630;p24;p25;p25-alpha;p25alpha ;TPPP;TPPP/p25;TPPP;TPPP1;Tubulin polymerization promoting protein;Tubulin polymerization-promoting protein |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies. |
| Concentration | 0.8 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Signal transduction |
| Conjugation | Unconjugated |
| Background | Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. |
Western blot analysis of Human fetal brain tissueusing TPPP Polyclonal Antibody at dilution of 1:250
Immunohistochemistry of paraffin-embedded Human liver cancer tissueusing TPPP Polyclonal Antibody at dilution of 1:25(×200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissueusing TPPP Polyclonal Antibody at dilution of 1:25(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
