TRAF6 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse; Rat
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Mouse heart Verified Samples in IHC:Human tonsil,Human ovarian cancer |
| Dilution | WB 1:500-1:2000;IHC 1:30-1:150 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of human TRAF6 |
| Abbre | TRAF6 |
| Synonyms | E3 ubiquitin-protein ligase TRAF6;Interleukin 1 signal transducer;Interleukin-1 signal transducer;MGC 3310;MGC:3310;MGC3310;OTTHUMP00000232772;OTTHUMP00000232773;RING finger protein 85;RNF 85;RNF85;TNF receptor associated factor 6;TNF receptor-associated factor 6;TNF receptor-associated factor 6;E3 ubiquitin protein ligase;TRAF 6;Traf6;TRAF6抗体 |
| Swissprot | |
| Calculated MW | 60 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Cytoplasm>cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. |
| Concentration | 0.54 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Cell Biology; Immunology; Signal Transduction |
| Conjugation | Unconjugated |
| Background | The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. |
Western blot analysis of Mouse heart tissue lysate using TRAF6 Polyclonal Antibody at dilution of 1:300
Immunohistochemistry of paraffin-embedded Human tonsil tissue using TRAF6 Polyclonal Antibody at dilution of 1:30(×200)
Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TRAF6 Polyclonal Antibody at dilution of 1:30(×200)
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Effect of IRAK1 on Apoptosis and Necroptosis of Hepatoma Cell Line SK-Hep1
IF: 2.265Journal：Chinese Medicine -
TRAF6 Affects RAC1 Expression and Apoptosis in SK-Hep1 Cells
IF: 1.802Journal：Chinese Medicine
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