VEGFA Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:293T Verified Samples in IHC:Human cervical cancer,Human brain |
Dilution | WB 1:1000-1:5000;IHC 1:30-1:150 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Synthetic peptide of human VEGFA |
Abbre | VEGFA |
Synonyms | Folliculostellate cell-derived growth factor;Glioma-derived endothelial cell mitogen;MGC70609;MVCD1;Vascular endothelial growth factor A;vascular endothelial growth factor A121;vascular endothelial growth factor A165;vascular endothelial growth factor;Vascular permeability factor;VEGF A;Vegf;VEGF-A;VEGF120;Vegfa;VEGFA;VPF抗体 |
Swissprot | |
Calculated MW | 27 kDa |
Observed MW | Refer to figures |
Cellular Localization | Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
Concentration | 1.8 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Cancer; Cardiovascular; Metabolism; Developmental Biology; Signal Transduction |
Conjugation | Unconjugated |
Background | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. |
Western blot analysis of 293T cell lysate using VEGFA Polyclonal Antibody at dilution of 1:1450
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using VEGFA Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using VEGFA Polyclonal Antibody at dilution of 1:60(×200)
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