ZAP70 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human; Mouse
- 应用性： WB;IHC
| Verified Samples |
Verified Samples in WB:Jurkat Verified Samples in IHC:Human thyroid cancer |
| Dilution | WB 1:500-1:2000;IHC 1:25-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide of human ZAP70 |
| Abbre | ZAP70 |
| Synonyms | 70 kDa zeta associated protein;70 kDa zeta-associated protein;EC 2.7.10.2;FLJ17670;FLJ17679;Selective T cell defect;SRK;STD;Syk related tyrosine kinase;Syk-related tyrosine kinase;Truncated ZAP kinase;Tyrosine protein kinase ZAP70;Tyrosine-protein kinase ZAP-70;TZK;ZAP 70;ZAP70;ZAP70;Zeta chain associated protein kinase 70kD;Zeta chain associated protein kinase 70kDa;Zeta chain associated protein kinase 70kDa isoform 1;Zeta chain associated protein kinase 70kDa isoform 2;Zeta chain of T cell receptor associated protein kinase 70;Zeta chain TCR associated protein kinase 70kD;Zeta chain TCR associated protein kinase 70kDa |
| Swissprot | |
| Calculated MW | 70 kDa |
| Observed MW | Refer to figures |
| Cellular Localization | Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes. |
| Concentration | 0.4 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer; Immunology; Signal Transduction; Tags and Cell Markers |
| Conjugation | Unconjugated |
| Background | Zeta-associated protein (ZAP)-70,a member of the Syk family of tyrosine kinases,plays an important role in T-cell receptor signaling,natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome. |
Western blot analysis of Jurkat cellsusing ZAP70Polyclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissueusing ZAP70Polyclonal Antibody at dilution of 1:50(×200)
| E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
| E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
| E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
| E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
| E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
| E-IR-R304A | Western Blot Detection Kit | 50Assays |
| E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |
