GAPDH Monoclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Mouse
- 反应性： Human;Mouse;Rat ;Bovine;Caprine;Guinea pig;Rabbit;Porcine;
- 应用性： WB;IF
| Verified Samples |
Verified Samples in WB:DU145,OS-RC-2,T-47D,HEC-1B,HepG2,HEK-293,Rat heart,Porcine brain,Bovine kidney,Guinea pig liver,Rabbit pancreas,Caprine spleen Verified Samples in IF: 293F |
| Dilution | WB 1:5000-10000 |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Abbre | GAPDH |
| Synonyms | aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component抗体 |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW | 36 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Purification Method | Protein A/G Purified |
| Research Areas | Cancer; Metabolism; Signal Transduction |
| Clone No. | 3B3 |
| Conjugation | Unconjugated |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR. |
Western Blot analysis of GAPDH in various cell lines at dilution of 1:5000.
Lane1: DU145 whole cell lysate, Lane2: OS-RC-2 whole cell lysate, Lane3:T-47D whole cell Lysates, Lane4: HEC-1B whole cell lysate, Lane5: HepG2 whole cell lysate, Lane6: HEK-293 whole cell lysate,. Lysates/proteins at 20g per lane.
Western blot analysis of GAPDH in various tissues of different species at dilution of 1:5000.
Lane1 : Rat heart, whole tissue lysate, Lane2: Porcine brain, whole tissue lysate, Lane3: Bovine kidney, whole tissue lysate, Lane4: Guinea pig liver, whole tissue lysate, Lane5: Rabbit pancreas, whole tissue ysate, Lane6: Caprine spleen, whole tissue lysate.
Lysates/proteins at 20g per lane.
Immunofluorescent analysis of 293F cells using anti-GAPDH monoclonal antibody at 1:400 dilution.
-
Identification and establishment of type IV interferon and the characterization of interferon-υ including its class II cytokine receptors IFN-υR1 and IL-10R2
IF: 16.6 -
Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion
IF: 14.593 -
PARP Inhibition Induces Synthetic Lethality and Adaptive Immunity in LKB1-Mutant Lung Cancer
IF: 11.2 -
A 3D bioprinted nano-laponite hydrogel construct promotes osteogenesis by activating PI3K/AKT signaling pathway
IF: 10.761 -
MnFe2O4 nanoparticles accelerate the clearance of mutant huntingtin selectively through ubiquitin-proteasome system
IF: 10.273Journal：BIOMATERIALS -
An ultralow dose of the NADPH oxidase inhibitor diphenyleneiodonium (DPI) is an economical and effective therapeutic agent for the treatment of colitis-associated colorectal cancer
IF: 8.579 -
Targeting Nrf2 with 3 H-1,2-dithiole-3-thione to moderate OXPHOS-driven oxidative stress attenuates IL-17A-induced psoriasis
IF: 7.419Journal：BIOMEDICINE & PHARMACOTHERAPY -
2-pentadecyl-2-oxazoline prevents cognitive and social behaviour impairments in the Amyloid β-induced Alzheimer-like mice model: Bring the α2 adrenergic receptor back into play
IF: 7.419 -
MANF ameliorates DSS-induced mouse colitis via restricting Ly6ChiCX3CR1int macrophage transformation and suppressing CHOP-BATF2 signaling pathway
IF: 7.169Journal：ACTA PHARMACOLOGICA SINICA -
Anti-Gouty Arthritis and Anti-Hyperuricemia Properties of Sanghuangporus vaninii and Inonotus hispidus in Rodent Models
IF: 6.706
