GAPDH Polyclonal Antibody
Price:
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:A431,HepG2,Hela,HT29,A549,Jurkat,MCF-7,Rat heart,Mouse brain,Mouse heart,Rat brain Verified Samples in IHC:Human bladder cancer,Human kidney,Mouse colon,Rat colon |
Dilution | WB 1:3000-1:6000;IHC 1:300-1:500 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein |
Abbre | GAPDH |
Synonyms | aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component |
Swissprot | |
Calculated MW | 35 kDa |
Observed MW | 35 kDa |
Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
Concentration | 0.66mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
Purification Method | Antigen Affinity Purification |
Research Areas | Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism |
Conjugation | Unconjugated |
Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. |
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