GAPDH Polyclonal Antibody
Price:
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC
Verified Samples |
Verified Samples in WB:A431,HepG2,Hela,HT29,A549,Jurkat,MCF-7,Rat heart,Mouse brain,Mouse heart,Rat brain Verified Samples in IHC:Human bladder cancer,Human kidney,Mouse colon,Rat colon |
Dilution | WB 1:3000-1:6000;IHC 1:300-1:500 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein |
Abbre | GAPDH |
Synonyms | aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component抗体 |
Swissprot | |
Calculated MW | 35 kDa |
Observed MW | 35 kDa |
Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
Concentration | 0.66mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
Purification Method | Antigen Affinity Purification |
Research Areas | Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism |
Conjugation | Unconjugated |
Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. |
-
Gallic Acid Ameliorates Aspergillus Fumigatus Keratitis Through Reducing Fungal Load and Suppressing the Inflammatory Response
IF: 4.925Journal:INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE -
K63 ubiquitin chains target NLRP3 inflammasome for autophagic degradation in ox-LDL-stimulated THP-1 macrophages
IF: 4.831 -
IL-36α Exerts Proinflammatory Effects in Aspergillus fumigatus Keratitis of Mice Through the Pathway of IL-36α/IL-36R/NF-κB
IF: 4.799 -
Isoforsythiaside Attenuates Alzheimer’s Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway
IF: 4.556Journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES -
Ultraviolet Radiation Promoted Hypoxia-Induced Apoptosis in HL-60 Human Promyelocytic Leukemia Cell Line
IF: 4.501 -
Laccase-mediated functionalization of natamycin by gallic acids for the therapeutic effect on Aspergillus fumigatus keratitis
IF: 4.432 -
Erythromycin reduces nasal inflammation by inhibiting immunoglobulin production, attenuating mucus secretion, and modulating cytokine expression
IF: 4.38 -
An Alternatively Spliced Variant of METTL3 Mediates Tumor Suppression in Hepatocellular Carcinoma
IF: 4.096 -
Biological effects on tooth root surface topographies induced by various mechanical treatments
IF: 3.973 -
FCN-A mediates the inflammatory response and the macrophage polarization in Aspergillus fumigatus keratitis of mice by activating the MAPK signaling pathway
IF: 3.943
E-AB-1003 | Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
E-AB-1043 | Streptavidin(peroxidase/HRP conjugated) | 500μL , 120μL , 60μL , 30μL , 1mL |
E-AB-1194 | HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed | 500μL , 120μL , 1mL |
E-IR-R217 | 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) | 18mL , 6mL , 3mL |
E-IR-R220 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) | 10mL , 3mL |
E-IR-R221 | Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) | 10mL , 3mL |
E-IR-R304A | Western Blot Detection Kit | 50Assays |
E-IR-R304B | High Accuracy and Absorbability Western Blot Detection Kit | 50Assays |