GFP Monoclonal Antibody
Price:
Size:
100μL
50μL
- 宿主： Mouse
- 反应性： All
- 应用性： WB;IF;IP
| Verified Samples |
Verified Samples in WB:293F cells transfected with the GFP-tag Verified Samples in IP: 293F cells transfected with the FLAG-GFP, 293F, anti-GFP-Tag monoclonal antibody |
| Dilution | WB 1:100000-200000;IF 1:8000-16000;IP 3ug/sample |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Immunogen | Recombinant GFP protein expressed by E.coli |
| Abbre | GFP-Tag |
| Synonyms | GFP;Green fluorescent protein抗体 |
| Swissprot | |
| Calculated MW | 32kDa |
| Observed MW | 32kDa |
| Concentration | 1mg/ml |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide,50% glycerol,pH 7.4 |
| Purification Method | Protein A/G Purified |
| Clone No. | 2A7 |
| Conjugation | Unconjugated |
| Background | The green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. It is first isolated from the jellyfish Aequorea victoria and is frequently used as a reporter of expression. GFP is widely used in gene expression, tracing and protein subcellualr localization. |
Western blot of GFP protein with anti-GFP at dilution of 1:50000, 1:100000, 200000. Lane 1:293F cells transfected with GFP-Tag fusion protein, lane 2: 293F cells transfected with GFP-Tag fusion protein, Lane 3:293F cells transfected with GFP-Tag fusion protein
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-GFP-Tag Monoclonal Antibody at dilution of 1:50000.
IP Result of 293F cells transfected with GFP-Tag fusion protein,using anti-GFP-Tag mouse monoclonal antibody. Lane 1:input, lane 2: mouse IgG2a Isotype Control, Lane 3: anti-GFP-Tag monoclonal antibody
