HA-Tag Monoclonal Antibody

货号：E-AB-48021
文献（2）
说明书

说明书

Price: ¥1280 ¥800 ¥499 ¥219

Size:

200μL 100μL 50μL 20μL

宿主： Mouse
反应性： All
应用性： WB;IF;IP;FCM

Verified Samples	Verified Samples in WB:HA-Tag fused recombinant protein Verified Samples in IF:293F Verified Samples in IP:293F Verified Samples in FCM:CHO
Dilution	WB 1:5000-10000;IP 3ug/sample;IF 1:400-800;FCM 0.2ug
Clonality	Monoclonal
Isotype	IgG2a
Immunogen	Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin.
Abbre	HA-Tag
Synonyms	HA epitope tag; HA1 ;HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain抗体
Calculated MW	28kDa
Observed MW	28kDa
Concentration	1mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Purification Method	Protein A/G Purified
Clone No.	5F7
Conjugation	Unconjugated
Background	The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.

Western blotting with anti-HA-Tag monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate

Immunofluorescent analysis of 293F cells transfected with HA-Tag fusion protein using anti-HA-Tag monoclonal antibody at dilution of 1:800.

IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag monoclonal antibody .lane 1: mouse IgG2a Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag mouse monoclonal antibody

1x106 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag mAb(E-AB-48021) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)

CYLD deficiency enhances metabolic reprogramming and tumor progression in nasopharyngeal carcinoma via PFKFB3

IF: 9.7

Journal：Cancer Letters

DOI：10.1016/j.canlet.2022.215586

PMID：35131382
The deubiquitinase OTUD1 noncanonically suppresses Akt activation through its N-terminal intrinsically disordered region

IF: 8.8

Journal：Cell Reports

DOI：10.1016/j.celrep.2022.111916

PMID：36640312

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