IDE Monoclonal Antibody
Price:
Size:
120μL
60μL
20μL
- 宿主： Mouse
- 反应性： Human
- 应用性： WB;IHC-p;IF
| Verified Samples |
Verified Samples in WB:Hela,HepG2 Verified Samples in IHC:Human liver cancer Verified Samples in IF:Human breast |
| Dilution | WB 1:500-1:2000;IHC 1:50-300;IF 1:100-1:300 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Immunogen | Synthetic Peptide |
| Abbre | IDE |
| Synonyms | Abeta-degrading protease;FLJ35968;Ide;IDE;Insulin protease;Insulin-degrading enzyme;Insulinase;Insulysin;OTTHUMP00000020097抗体 |
| Swissprot | |
| Observed MW | 118kDa |
| Cellular Localization | Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification Method | Protein A purification |
| Research Areas | Cancer; Cell Biology; Cardiovascular; Metabolism; Neuroscience; Signal Transduction |
| Clone No. | Clone:1H4 |
| Conjugation | Unconjugated |
| Background | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified. |
Western Blot analysis of 1) Hela, 2) HepG2 cells using IDE Monoclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using IDE Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human breast tissue using IDE Monoclonal Antibody at dilution of 1:200.
