AKT Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： IHC;IF
| Verified Samples |
Verified Samples in IHC:Human mammary cancer,Human uterine cancer,Human tonsil,Human lung cancer Verified Samples in IF:L929 |
| Dilution | IHC 1:50-1:100;IF 1:50-1:100 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human AKT (NP_005154.2). |
| Abbre | AKT |
| Synonyms | AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1抗体 |
| Swissprot | |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. |
| Concentration | 1mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Epigenetics and Nuclear Signaling; Metabolism; Signaling transduction |
| Conjugation | Unconjugated |
| Background | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. |
Immunohistochemistry of paraffin-embedded Human mammary cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human uterine cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using AKT Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using AKT Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
