CYCS Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat
- 应用性: WB;IHC;IF
Verified Samples |
Verified Samples in WB:Hela,HepG2,Mouse lung,Mouse brain,Rat brain Verified Samples in IHC:Human lung cancer,Human heart Verified Samples in IF:Mouse kidney,Mouse liver |
Dilution | WB 1:500-1:2000;IHC 1:500-1:1000;IF 1:200-1:800 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Cytochrome c |
Abbre | CYCS |
Synonyms | Cytochrome C; Somatic;CYC;Cytochrome C;THC4;HCS.抗体 |
Swissprot | |
Calculated MW | 12kDa |
Observed MW | 12kDa |
Cellular Localization | Mitochondrion |
Concentration | 1.5 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Cell Biology; Cardiovascular; Metabolism; Signal Transduction |
Conjugation | Unconjugated |
Background | Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation,cytochrome c can be released from mitochondria into cytoplasm,resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition,cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy. |
Western Blot analysis of various samples using CYCS Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using CYCS Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of paraffin-embedded mouse kidney using CYCS Polyclonal Antibody at dilution of 1:500.
Immunofluorescence analysis of paraffin-embedded mouse liver using CYCS Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry analysis of paraffin-embedded Human Heart using CYCS Polyclonal Antibody(Elabscience Product Detected by Lifespan).
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and caspase 3 levels in rats with myocardial infarction
IF: 1.246Journal:CHEMICAL PAPERS