EIF2 alpha Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主: Rabbit
- 反应性: Human;Mouse;Rat;Monkey
- 应用性: WB;IHC-p;IF
Verified Samples |
Verified Samples in WB:MCF-7,A549,K562,HEK293 Verified Samples in IHC:Human liver cancer Verified Samples in IF:Rat kidney |
Dilution | WB 1:500-1:2000;IHC 1:100-1:300;IF 1:50-1:200 |
Clonality | Polyclonal |
Isotype | IgG |
Immunogen | Synthesized peptide derived from human eIF2α around the non-phosphorylation site of Ser51. |
Abbre | eIF2α |
Synonyms | EIF 2 alpha;EIF 2;EIF 2A;EIF 2alpha;eIF-2-alpha;eIF-2A;EIF-2alpha;EIF2 alpha;EIF2;EIF2A;EIF2S1;Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa;Eukaryotic translation initiation factor 2 subunit 1 alpha;Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;IF2A抗体 |
Swissprot | |
Calculated MW | 36kDa |
Observed MW | 38kDa |
Cellular Localization | Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). |
Concentration | 1 mg/mL |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
Purification Method | Affinity purification |
Research Areas | Cancer; Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Background | Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. |
Western Blot analysis of various cells using EIF2 alpha Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat kidney tissue using EIF2 alpha Polyclonal Antibody at dilution of 1:200.