Flag-Tag Polyclonal Antibody
Price:
Size:
200μL
100μL
50μL
20μL
- 宿主： Rabbit
- 反应性： All
- 应用性： WB;IF;IP
| Verified Samples |
Verified Samples in WB:Flag-Tagged fusion protein Verified Samples in IP: Hela, 293F |
| Dilution | WB 1:2000-5000;IP 3ug/sample;IF 1:6000-12000 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to Flag tag conjugated to keyhole limpet haemocyanin. |
| Abbre | Flag-Tag |
| Synonyms | DDDDK epitope tag;DDDDK epitope tag抗体 |
| Calculated MW | 28kDa |
| Observed MW | 28kDa |
| Concentration | 4mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol,pH 7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Background | FLAG-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. This |
Western blotting with Anti-FLAG rabbit polyclonal antibody at dilution of 1:1000.Lane1: FLAG tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of Hela cells transfected with the plasmid overexpressing Flag fusion Protein, using anti-Flag -Tag polyclonal antibody at 1:200 dilution.
Immunofluorescent analysis of 293F cells transfected with the FLAG-GFP, using anti-Flag-Tag Polyclonal Antibody at dilution of 1:12000.
IP Result of 293F cells transfected with FLAG-Tag fusion protein,using anti-FLAG-Tag rabbit antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-FLAG-Tag rabbit antibody
