HSP27 Polyclonal Antibody
Price:
Size:
200μL
120μL
60μL
20μL
- 宿主： Rabbit
- 反应性： Human;Mouse;Rat
- 应用性： WB;IHC-p;IF
| Verified Samples |
Verified Samples in WB:22RV1 Verified Samples in IHC:Human stomach cancer Verified Samples in IF:Mouse kidney |
| Dilution | WB 1:500-1:2000;IHC 1:100-1:300;IF 1:50-1:200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human HSP27 around the non-phosphorylation site of Ser15. |
| Abbre | HSP27 |
| Synonyms | Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;epididymis secretory protein Li 102;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;heat shock protein family B (small) member 1;HEL-S-102;HMN2B;HS.76067;Hsp 25;HSP 27;Hsp 28;Hsp B1;Hsp25;HSP27;Hsp28;HspB1;HSPB1;SRP27;Stress responsive protein 27;Stress-responsive protein 27抗体 |
| Swissprot | |
| Calculated MW | 23kDa |
| Observed MW | 27kDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton>spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. |
| Concentration | 1 mg/mL |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer; Cardiovascular; Signal Transduction |
| Conjugation | Unconjugated |
| Background | Involved in stress resistance and actin organization. |
Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human stomach cancer tissue using HSP27 Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Mouse kidney tissue using HSP27 Polyclonal Antibody at dilution of 1:200.
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IF: 5.115
