RAF1 Polyclonal Antibody

货号：E-AB-60020
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说明书

说明书

Price: ¥3580 ¥2220 ¥1380

Size:

200μL 120μL 60μL

宿主： Rabbit
反应性： Human;Mouse;Rat
应用性： WB;IHC;IF

Verified Samples	Verified Samples in WB:293T,Jurkat,HeLa,MCF-7,HepG2,Mouse liver Verified Samples in IHC:Rat liver,Rat heart Verified Samples in IF:U2OS
Dilution	WB 1:500-1:2000;IHC 1:50-1:200;IF 1:50-1:200
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein of human RAF1 (NP_002871.1).
Abbre	RAF1
Synonyms	CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1抗体
Swissprot	P04049
Calculated MW	73kDa/75kDa
Observed MW	73kDa
Cellular Localization	Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes.
Concentration	1mg/mL
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Buffer	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method	Affinity purification
Research Areas	Cancer; Cell Biology; Metabolism; Signal Transduction
Conjugation	Unconjugated
Background	This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Western blot analysis of extracts of various cell lines using RAF1 Polyclonal Antibody at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded Rat liver using RAF1 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded Rat heart using RAF1 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using RAF1 Polyclonal Antibody

IL-35 enhances angiogenic effects of small extracellular vesicles in breast cancer

IF: 5.542

Journal：FEBS Journal

DOI：10.1111/febs.16359

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